Theerlenmeyer shake botolondi wapadera chikhalidwe chidebe cha kuyimitsidwa selo chikhalidwe, ndipo angagwiritsidwenso ntchito kukonzekera, kusakaniza ndi kusunga TV zosiyanasiyana.Izi consumable chikhalidwe ntchito pamene culturing Jurkat maselo.

Maselo a Jurkat amachokera ku magazi ozungulira a mnyamata wazaka 14 ndipo ndi selo loyimitsidwa.Ma cell opangidwa ndi Jurkat omwe alibe majini ena amapezeka kale m'mabanki a cell culture.Mizere ya T lymphocyte yosafa yomwe imagwiritsidwa ntchito kwambiri pofufuza pachimake T cell khansa ya m'magazi, ma cell a T cell signing, ndi ma chemokine receptors omwe amatha kutenga kachilomboka, makamaka mafotokozedwe a HIV.Lili ndi ntchito zambiri zofufuza zamoyo, monga kugwiritsa ntchito maselo a Jurkat kuti aphunzire M1-RNA ya ribonuclease P, ndi kuphunzira kwa M1-RNA ya anti-MHC class II transcriptal activator (CIITA) kuti aletse. kufotokoza kwa mamolekyu a MHC kalasi yachiwiri pa selo.

Pamene culturing Jurkat maselo mu erlenmeyer kugwedeza flasks, RPMI1640 sing'anga, 10% FBS chofunika;kutentha kumayendetsedwa pa 37 ° C, 5% carbon dioxide, PH mtengo 7.2-7.4, aseptic nthawi zonse kutentha chikhalidwe.Pukutani ndikuphera tizilombo toyambitsa matenda ndi 75% mowa musanasamutsire ku benchi yoyera kwambiri, chotsani cell cryovial mu thanki ya nayitrogeni yamadzimadzi, nthawi yomweyo ikani mumadzi osambira a 37 ° C ndikugwedezani cell cryotube kuti isungunuke mwachangu.Kenako, pambuyo centrifugation, pipetting ndi kusanganikirana, etc., izo anaikidwa selo chofungatira kuti kulima.

Maselo amakhudzidwa kwambiri ndi chilengedwe.Pokulitsa ma cell a Jurkat mu erlenmeyer cell shake flasks, ukhondo wamunthu uyenera kuchitidwa bwino, ma reagents osabala ayenera kugwiritsidwa ntchito, ndipo mfundo za ntchito ya aseptic ziyenera kutsatiridwa kuti tipewe kuyambitsa mabakiteriya komanso kukhudza kukula kwa maselo.