PETG chikhalidwe sing'anga botolondi botolo lapulasitiki logwiritsidwa ntchito kwambiri.Thupi lake la botolo limakhala lowonekera kwambiri, limatenga mawonekedwe a square, kulemera kwake, ndipo sikophweka kusweka.Ndi chidebe chabwino chosungira.Zomwe timagwiritsa ntchito nthawi zambiri zimakhala zitatu izi:

1. Seramu: Seramu imapereka maselo okhala ndi zakudya zofunikira, kukula, mapuloteni omangirira, ndi zina zotero, kupewa kuwonongeka kwa makina ku maselo, komanso kuteteza maselo mu chikhalidwe.Seramu yosungirako nthawi yayitali iyenera kusungidwa m'malo otentha otsika a -20°C mpaka -70°C.Ngati asungidwa mufiriji ya 4 ° C, nthawi zambiri osapitilira mwezi umodzi.

2.Culture sing'anga: Sing'anga ya chikhalidwe nthawi zambiri imakhala ndi chakudya, zinthu za nayitrogeni, mchere wachilengedwe, mavitamini ndi madzi, ndi zina. Sizinthu zokha zomwe zimapatsa ma cell zakudya komanso kulimbikitsa kuchuluka kwa maselo, komanso malo okhala ma cell ndi kuberekana. .Malo osungiramo sing'anga ndi 2 ° C-8 ° C, otetezedwa ku kuwala.

3. Osiyanasiyana reagents: Kuwonjezera kusungirako seramu ndi sing'anga chikhalidwe, PETG sing'anga mabotolo angagwiritsidwenso ntchito monga zotengera zosungira zosiyanasiyana zamoyo reagents, monga buffers, reagents dissociation, mankhwala, njira cryopreservation cell, madontho zothetsera, Kukula zowonjezera, etc. Ena mwa ma reagentswa amafunika kusungidwa pa -20 ° C, pamene ena amasungidwa kutentha.Ziribe kanthu kuti ndi malo otani, botolo lapakati limatha kukwaniritsa zofunikira zawo zosungirako.

PETG sing'anga botolo zimagwiritsa ntchito kugwira pamwamba njira zitatu.Pofuna kuwongolera kuwonetsetsa kwa kuchuluka kwa yankho, pali sikelo pa thupi la botolo.Mayankho omwe ali pamwambawa amagwiritsidwa ntchito mu chikhalidwe cha ma cell, ndipo chidwi chiyenera kuperekedwa ku ntchito ya aseptic powonjezera.