Mu kuyimitsidwa cell culture,cell kugwedeza botolondi mtundu wa cell chikhalidwe consumable.Kukula kwa maselo oyimitsidwa sikunadalire pamwamba pa zinthu zothandizira ndipo adakula mu chikhalidwe choyimitsidwa mu chikhalidwe cha chikhalidwe.Kodi timadziwa bwanji kuchuluka kwa madzi oti tiwonjezere pa chikhalidwe chenicheni?

Zodziwika bwino za cell shaker zimaphatikizapo 125ml, 250ml, 500ml ndi 1000ml kuti akwaniritse zosowa zamitundu yosiyanasiyana yama cell.Mwachitsanzo, mabotolo a 125ml ndi 250ml omwe ali ndi mphamvu zochepa amagwiritsidwa ntchito makamaka pazoyesera zazing'ono, pamene 500ml ndi 1000ml zomwe zimagwiritsidwa ntchito zimagwiritsidwa ntchito poyesa chikhalidwe cha maselo apakati.Mukamagwiritsa ntchito zinthu zamtunduwu, kugwedezeka kwa shaker kuyenera kugwiritsidwa ntchito kuchepetsa kuchuluka kwa ma cell agglomeration ndikusunga kukula bwino kwa maselo.Chikhalidwe cha ma cell chikuyenera kuchitika m'malo owuma.Choncho, botolo la chikhalidwe cha makona atatu lidzatsekedwa mwapadera lisanayambe kugwiritsidwa ntchito kuti likwaniritse zotsatira za palibe DNase, palibe enzyme ya RNA, komanso palibe zigawo zotengedwa ndi zinyama, zomwe zimapereka malo abwino oti ma cell akule.

Malinga ndi mafotokozedwe osiyanasiyana a botololo, kuchuluka kokwanira kodzaza kwazinthu zinayi za botolo kuchokera pansi mpaka pamwamba ndi 30ml, 60ml, 125ml, 500ml.Nthawi zambiri, kuchuluka kwa yankho mu chikhalidwe cha maselo kumayendetsedwa pafupifupi 20% -30% ya voliyumu yonse ya botolo logwedezeka, ndipo pali mzere womveka bwino pa botolo la botolo kuti lithandizire kuwona mphamvu ya yankho. .

Zomwe zili pamwambazi ndi kuchuluka kwamadzimadzi komwe kumawonjezeredwa kumitundu yosiyanasiyana ya cell shaker, yomwe siinakhazikike.Kuthekera kuyenera kuganiziridwa mozama molingana ndi mawonekedwe a kukula kwa maselo ndi kachulukidwe ka inoculation, kuti tipewe kutengera kukula kwa maselo chifukwa cha kuchuluka kwamadzimadzi komwe kumawonjezeredwa.