Ma cell vacuolation amatanthauza mawonekedwe a vacuoles (vesicles) amitundu yosiyanasiyana mu cytoplasm ndi phata la maselo osokonekera, ndipo maselo ndi ma cell kapena reticular.Pali zifukwa zambiri za vutoli.Tikhoza kuchepetsa vacuolation wa maselo mucell Culture botolopang'ono momwe ndingathere kupyolera mu ntchito za tsiku ndi tsiku.

1. Tsimikizani mawonekedwe a selo: dziwani momwe maselo alili musanayambe kukulitsa maselo, ndipo yesetsani kusankha maselo omwe ali ndi chiwerengero chapamwamba kwambiri kuti alimidwe, kuti mupewe vacuoles chifukwa cha ukalamba wa maselo panthawi ya kulima.

2. Dziwani mtengo wa pH wa chikhalidwe cha chikhalidwe: kutsimikizira kuyenerera kwa pH ya chikhalidwe cha chikhalidwe ndi pH yofunikira ndi maselo kuti asakhudze kukula kwa maselo chifukwa cha pH yosayenera.

3. Yang'anirani nthawi ya trypsin digestion: mukakhala subculture, sankhani kuchuluka koyenera kwa trypsin ndikusankha nthawi yoyenera kugaya chakudya, ndipo pewani kutulutsa mpweya wambiri panthawi ya opareshoni.

4. Yang'anani momwe maselo alili nthawi iliyonse: Mukamapanga ma cell, onani momwe maselo alili mucell Culture botolonthawi iliyonse kuonetsetsa kuti maselo amafunika zakudya zokwanira ndi kupewa selo vacuolization chifukwa chosowa michere.

5. Yesetsani kugwiritsa ntchito seramu ya fetal bovine yokhala ndi njira zabwino komanso zokhazikika, chifukwa seramu yotereyi imakhala ndi michere yambiri ndipo imakhala ndi zinthu zochepa zomwe zimapatsa chidwi, zomwe zimatha kupewa zovuta zotere.

The pamwamba ntchito akhoza kuchepetsa vacuolation wa maselo mucell Culture botolo.Kuphatikiza apo, zofunikira za sterility ziyenera kutsatiridwa mosamalitsa panthawi ya opareshoni kuti kuchepetsa kuthekera kwa kuipitsidwa kosiyanasiyana.Ngati ma cell apezeka kuti ali ndi kachilombo, amayenera kutayidwa munthawi yake kuti asakhudze zoyeserera zomwe zikubwera.

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