Kamodzi maselo ife chikhalidwe mu

cell fakitaleali ndi kachilombo, ambiri a iwo ndi ovuta kuwagwira.Ngati maselo oipitsidwa ali amtengo wapatali komanso ovuta kuwapezanso, njira zotsatirazi zingagwiritsidwe ntchito kuwachotsa.

1. Gwiritsani ntchito maantibayotiki

Maantibayotiki amatha kupha mabakiteriyamafakitale a cell.Mankhwala ophatikiza ndi othandiza kuposa mankhwala okha.Mankhwala oteteza ndi othandiza kwambiri kuposa mankhwala atatha kuipitsidwa.Mankhwala oteteza nthawi zambiri amagwiritsa ntchito maantibayotiki awiri (penicillin 100u/mL kuphatikiza streptomycin 100μg/mL).Pambuyo pa kuipitsidwa, njira yoyeretsera iyenera kukhala 5 mpaka 10 kuposa kuchuluka kwanthawi zonse.Mankhwalawa ayenera kugwiritsidwa ntchito kwa maola 24 mpaka 48 mutatha kuwonjezera, ndiyeno m'malo mwachizolowezi.Culture madzimadzi.Njirayi ingakhale yothandiza kumayambiriro kwa kuipitsidwa.Kuphatikiza pa penicillin ndi streptomycin, maantibayotiki omwe amagwiritsidwa ntchito amathanso kukhala gentamicin, kanamycin, polymyxin, tetracycline, nystatin, ndi zina zotero. Zomwe zimagwiritsidwa ntchito kwambiri ndi 400 mpaka 800 μg / mL kanamycin kapena 200 μg / mL tetracycline.Sing'anga imasinthidwa masiku awiri mpaka atatu aliwonse ndikuperekedwa kwa mibadwo 1 mpaka 2 kuti alandire chithandizo.M'zaka zaposachedwa, zanenedwa kuti 4-fluoro, 2-hydroxyquinoline (Ciprofloxacin, Cip), Pleu-romutilin derivative (Pleu-romutilin derivative, BM-Cyclin2: BM-1 ndi tetracycline derivative (BM-2)) Maantibayotiki ndi yothandiza kupha mycoplasma ikagwiritsidwa ntchito yokha kapena kuphatikiza.Maantibayotiki atatuwa onse amakonzedwa kukhala 250X concentrated solutions mu PBS ndipo amasungidwa pa -20°C kuti agwiritsidwe ntchito mtsogolo.Kugwiritsidwa ntchito kwa Cip ndi 10 μg/mL, BM-1 ndi 10 μg/mL, ndi BM-2 ndi 5μg/mL.Mukamagwiritsa ntchito, choyamba funani sing'anga yoipitsidwa, onjezani RPMI1640 sing'anga yomwe ili ndi BM-1, kenako funani sing'anga yachikhalidwe pakatha masiku atatu, onjezani RPMI1640 sing'anga yokhala ndi BM-2, ndi chikhalidwe kwa masiku 4, ndi zina zotero kwa masiku atatu otsatizana. .kuzungulira, mpaka kutsimikiziridwa ndi 33258 fulorosenti kudetsa microscopy kuti mycoplasma yathetsedwa, ndiye yachibadwa sing'anga chikhalidwe anawonjezera kwa chikhalidwe ndi ndime 3-4 zina.

2. Chithandizo cha kutentha

Kulowetsa chikhalidwe cha minofu yoipitsidwa pa 41 ° C kwa maola 18 kumatha kupha mycoplasma, koma kumakhala ndi zotsatira zoyipa pama cell.Choncho, kuyesedwa koyambirira kuyenera kuchitidwa musanayambe chithandizo kuti mufufuze nthawi yotentha yomwe imatha kupha mycoplasma mpaka kufika pamtunda waukulu komanso kukhala ndi zotsatira zochepa pa maselo.Njira imeneyi nthawi zina imakhala yosadalirika.Ngati chithandizo ndi mankhwala choyamba ndi kutentha pa 41 ° C, zotsatira zake zidzakhala bwino.

3. Gwiritsani ntchito seramu yeniyeni ya mycoplasma

Kuwonongeka kwa Mycoplasma kumatha kuchotsedwa ndi 5% ya kalulu mycoplasma immune serum (hemagglutination titer 1:320 kapena pamwambapa).Chifukwa chakuti antibody yeniyeniyo imatha kulepheretsa kukula kwa mycoplasma, imasintha pakadutsa masiku 11 mutalandira chithandizo cha antiserum ndipo imakhalabe yopanda pake pakadutsa miyezi isanu.ndi negative.Komabe, njirayi ndi yovuta kwambiri komanso si yabwino komanso yotsika mtengo monga kugwiritsa ntchito maantibayotiki.

4. Njira zina

Kuphatikiza pa njira zomwe tatchulazi zochotsera kuipitsidwa, palinso njira zothirira ndi kubala nyama, njira za macrophage phagocytosis, njira zowonjezera bromouracil ku zoipitsidwa.mabotolo chikhalidwendiyeno kuwayatsa ndi kuwala, ndi njira zosefera, ndi zina zotero, koma zonse zimakhala zovuta komanso zosagwira ntchito.Choncho, pamene kuipitsidwa kwa mycoplasma kumachitika, pokhapokha ngati kuli kofunikira kwambiri, nthawi zambiri kumatayidwa ndikukonzedwanso.

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