Tikamagwiritsa ntchito zinthu zina zama cell cell, timakumana ndi vuto la cell cell.Lero, ndikugawana mwachidule momwe mungagwiritsire ntchito ma flasks apamwamba kwambiri pama cell.Pamene ife ntchito ma flasks apamwamba kwambiri ogwedeza(https://www.luoron.com/3l5l-high-efficiency-erlenmeyer-flask-product/) kwa selo ndimeyi, pali njira ziwiri zomwe mungasankhe, monga kusonkhanitsa maselo ndi centrifugation ndiyeno ndime, kapena kutsogolera. ndime.

Njira ya Centrifugal:

(1) Kusamutsa ma cell mushake botolo lapamwamba kwambiri pamodzi ndi chikhalidwe sing'anga ku chubu centrifuge kwa centrifugation.

(2) Tayani zamatsenga, onjezani chikhalidwe chatsopano chubu cha centrifuge ndipipettekupanga cell kuyimitsidwa.

(3) Werengani ndi kuika mu fulakisi ya chikhalidwe chatsopano motsatira.

Ngati ndime yachindunji ikuvomerezedwa, lolani maselo oimitsidwawo akhazikike pang'onopang'ono pansi pa botolo lapamwamba logwedezeka, kuyamwa 1/2 ~ 2/3 ya supernatant, ndiyeno pipette kupanga kuyimitsidwa kwa selo musanayambe ndimeyi.

Zomwe tikuyenera kulabadira panthawi ya opaleshoni ndikuti trypsin iyenera kutenthedwa kale, ndipo kutentha kumakhala pafupifupi 37 ° C.Kuthamanga kwa centrifugation kuyenera kukhala koyenera.Ngati liwiro liri lotsika kwambiri, maselo sangathe kulekanitsidwa bwino.Ngati liwiro la centrifugation ndi lalitali kwambiri ndipo nthawi yayitali kwambiri, maselo amafinyidwa, kuwononga kapena kufa.Maselo amayenera kuwonedwa pafupipafupi, ndipo ngati kuipitsidwa kwapezeka, kuyenera kuthetsedwa munthawi yake.