Chikhalidwe cha cell ndimeyi chimatanthawuza njira yogawa chikhalidwecho m'zigawo zing'onozing'ono ndikuchiyikanso mu chotengera china cha chikhalidwe (botolo) kuti chiwonjezere chikhalidwe.Ma cell shaker apamwamba kwambirindi wamba consumable kwa kuyimitsidwa cell chikhalidwe, ndiye mmene ntchito mkulu dzuwa shaker kwa selo ndime?

Mwachilengedwe chawo, ma cell oyimitsidwa satsatira, kotero palibe ma enzyme omwe amafunikira kuti awachotse pamwamba pa shaker yogwira ntchito kwambiri.Mu labotale wamba, ndime yolunjika ndi ndime ya centrifugal imagwiritsidwa ntchito podutsa ma cell oyimitsidwa.Maselo akamaonedwa kuti ndi ochuluka kwambiri (ma cell suspension amasanduka achikasu), maselo amakhala okonzeka kudutsa.

Ngati maselo akukula bwino pansi pa maikulosikopu, njira yolunjika ingagwiritsidwe ntchito.The medium mubotolo logwedeza bwino kwambiriadagawidwa molingana ndi botolo la chikhalidwe chatsopano, ndipo sing'anga yatsopano idawonjezedwa.Kuchulukana kwa maselo kunawonedwa tsiku lotsatira kuti adziwe ngati madziwa akufunika.

Ngati selo silikuyenda bwino, njira ya centrifugal iyenera kugwiritsidwa ntchito.Choyamba, kuyimitsidwa kwa selo kumasamutsidwa kuchubu cha centrifuge, centrifuged pa 1000rpm kwa 5 min, ndiye supernatant imatayidwa, cell precipitates imabalalika pang'onopang'ono, ndipo maselo amayimitsidwanso ndi sing'anga yatsopano.Pomaliza, yoyenera kuchuluka kwa selo kuyimitsidwa ndi odzipereka ndi udzu, anaika mu latsopanochikhalidwe botolo, ndipo kuchuluka koyenera kwa sing'anga yatsopano kumawonjezeredwa.Pitirizani kulima.

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