Liquid strain ndi mtundu womwe umakulitsidwa ndi chikhalidwe chamadzimadzi.Zili ndi ubwino wa nthawi yochepa yopanga zovuta, msinkhu wa bakiteriya wosasinthasintha, inoculation yabwino, yoyenera kupanga fakitale, ndi zina zotero.Yalandiridwa ndi alimi ambiri.Cell shaker (https://www.luoron.com/plastic-erlenmeyer-flask-with-vent-cap-2-product/) ndi mtundu wa zinthu consumable zofunika chikhalidwe cha madzi tizilombo.Mfundo zotsatirazi ziyenera kutsatiridwa mu chikhalidwe:

1.Kukonzekera sing'anga chikhalidwe molingana ndi chilinganizo cha zigawo zosiyanasiyana, mu chidebe, kupasuka ndi madzi, ogaŵikana mabotolo triangular, kawirikawiri 250 ~ 300mL selo kugwedeza botolo 50mL sing'anga chikhalidwe,500mL katatu botolo  (https://www.luoron.com/plastic-erlenmeyer-flask-with-vent-cap-2-product/)100mL chikhalidwe sing'anga, pulagi thonje pulagi, ndi nyuzipepala wokutidwa;Itha kupangidwanso ndi zigawo 8 za chosindikizira cha 8cm × 8cm botolo la botolo, kunja ndi kusindikiza pepala lofiirira.
2. Njira yoletsa kutsekereza ya Autoclave nthawi zambiri imatengedwa kuti ikhale yotseketsa, yomwe imafunika 121 ℃ ndi 30min.Chotsani ndikuzizira mpaka 30 ℃.Ikani mu chipinda chosabala kapena bokosi loyikiramo kuti mugwiritse ntchito.
3. Malingana ndi zofunikira za opaleshoni ya aseptic, botolo lirilonse likhoza kulumikizidwa ndi 2 ~ 3cm2 slant strain, ndipo mtundu uliwonse wa slant ukhoza kulumikizidwa ndi mabotolo 4 ~ 5.Ndi bwino kubweretsa sing'anga ndi zina zowonjezera, kuti azitha kuyandama pamwamba pa sing'anga.Pambuyo pa katemera, kutseka pakamwa pa botolo ndi yopyapyala poyamba plugged, ndi kulimanga mwamphamvu ndi chingwe.
4. Botolo la jekeseni lopangidwa likhoza kuikidwa pabedi logwedezeka chifukwa cha chikhalidwe, kapena likhoza kuyikidwa mu kutentha kosalekeza kwa 24 ~ 26 ℃ kwa 48h, ndiyeno chikhalidwe cha oscillated chikhoza kuchitidwa pambuyo poti aerogenic mycelium ipitirire ku chikhalidwe cha chikhalidwe. .Kuthamanga kwafupipafupi kwa shaker yobwerezabwereza ndi 80 ~ 120r / min, ndipo ya shaker yozungulira ndi 150 ~ 220r / min.3 ~ 4d imatha kukulitsidwa mu shaker.Kumapeto kwa chikhalidwe, chifukwa cha mitundu yosiyanasiyana, sing'anga chikhalidwe anaonekera mitundu yosiyanasiyana, monga oyisitara bowa, enoki bowa chikhalidwe sing'anga anali kuwala chikasu;Bowa, ericius erinaceus chikhalidwe sing'anga anali wofiira bulauni, ndi fungo la bowa;Chikhalidwe cha bowa ndi bulauni, wandiweyani komanso wotsekemera.Sing'anga ya chikhalidwe imatha kugwiritsidwa ntchito kupanga kapena kukulitsa chikhalidwe pokhapokha ngati kulemera kowuma kwa mycelium kufika 10g/L ndipo m'mimba mwake mwa pellets ya mycelium ndi 1 ~ 2mm.
 
5. Secondary madzi kupsyinjika kupanga yachiwiri madzi kupsyinjika chikhalidwe sing'anga kupanga ofanana mlingo wa madzi kupsyinjika, chikhalidwe chidebe ayenera kukhala wamkulu, mphamvu si oposa 3500mL.Pambuyo potseketsa ndi kuziziritsa, mitundu yamadzimadzi yofufumitsa imayikidwamo5L cell shaker(https://www.luoron.com/3l5l-high-efficiency-erlenmeyer-flask-product/）pa chiŵerengero cha 5% -10% ndi chikhalidwe pa shaker pa liwiro pang'onopang'ono.Patapita nthawi oscillating chikhalidwe, madzi tizilombo toyambitsa matenda ndi yunifolomu kufalitsa microspheres ndi bwino nayonso mphamvu madzi akhoza analandira.
Mwachidule, pamene ntchito selo shaker kuti chikhalidwe madzi tizilombo toyambitsa matenda, ntchito molingana ndi njira pamwamba, ndi kulabadira kulamulira kutentha, kugwedeza pafupipafupi ndi zinthu zina mu ndondomeko kuonetsetsa chikhalidwe zotsatira.
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