Kukula kwa ma cell kumakhala ndi zofunika kwambiri pa chilengedwe, kutentha, mtengo wa PH, ndi zina zotero, ndipo mtundu wa zinthu zomwe zimagwiritsidwa ntchito mu cell zikhudzanso kukula kwa maselo.Fakitale yama cellndi ambiri consumable kwa adherent cell chikhalidwe, ndipo ubwino wake makamaka amakhudzidwa ndi zinthu zinayi.

1, kupanga zipangizo: mkulu khalidwe zopangira ndi maziko a mankhwala apamwamba, selo fakitale zopangira polystyrene (PS), ndipo ayenera kutsatira zofunika za USP Class VI mlingo, mawu kuyesa zinthu pulasitiki. m'zachipatala ndi mankhwala a mapaipi muzogwiritsira ntchito biomedical zoyesa mozama kwambiri, zimagwirizana ndi zomwe sizinali zachipatala za labotale.

2, malo opangira: ma cell amakhudzidwa kwambiri ndi malo omwe akukula, chifukwa chake pamafunika kuti zinthu zomwe zimagwiritsidwa ntchito zisakhale ndi endotoxin ndi zinthu zina zovulaza m'maselo, zomwe zimayika patsogolo zofunikira pakupanga malo.Zomwe zimagwiritsidwa ntchito ziyenera kupangidwa m'chipinda choyeretsera zikwi khumi, ndipo zidzatsimikiziridwa mozama (kuzindikira plankton, sedimentation bacteria ndi particles suspended).Kuwongolera kwaubwino kudzachitika molingana ndi msonkhano wa GMP kuti zitsimikizire kukhazikika kwa chilengedwe.

3, kuwongolera njira zopangira: izi zimatanthawuza zomwe zimapangidwa popanga ulalo uliwonse kuphatikiza, monga magawo a jakisoni, kutentha kwa jekeseni, ndi zina zotere, zomwe zimakhudza mtundu wa chinthu chomalizidwa.

4, kuyang'anira khalidwe: kuyang'anira khalidwe ndi njira yofunikira mukamaliza kupanga fakitale yama cell, zinthu zomwe ziyenera kuyesedwa zimaphatikizapo kusindikiza, chitetezo chachilengedwe, chitetezo chakuthupi ndi mankhwala, kutsimikizira kutsimikizika kwazinthu, hydrophilicity pamwamba, ndi zina zotero, kupyolera mu mayesero awa kuti mudziwe. kaya zinthuzo zikukwaniritsa miyezo yabwino.

Zinthu zomwe zimakhudza ubwino wa mafakitale a maselo makamaka zimaphatikizapo mbali zinayi zomwe zili pamwambazi.Pokhapokha poyang'anira zinthu izi bwino tikhoza kupanga zinthu zamtengo wapatali ndipo motero timapereka malo abwino oti maselo akule.