Gwirani chikhalidwe cha botoloali mu siteji ya kupsyinjika kupsyinjika ndi chikhalidwe (woyendetsa mayeso), zikhalidwe chikhalidwe ayenera kukhala pafupi kwambiri ndi mmene nayonso mphamvu kupanga chikhalidwe chikhalidwe, ntchito yaikulu, nthawi yaitali, ntchito zovuta.Zinthu zimene zimakhudza dzuwa mkulu wa kugwedeza botolo chikhalidwe makamaka chikhalidwe kutentha, kugwedeza matalikidwe a shaker, kuchuluka kwa kugwedeza botolo, pH wa sing'anga chikhalidwe, mamasukidwe akayendedwe a sing'anga, etc. Culture kutentha: mycelium kukula kutentha Mitundu yosiyanasiyana ya bowa ndi yosiyana, kutentha kwakukulu kwa kukula kwapakati pa 22 ℃ ndi 30 ℃, ngati kutentha kwa chikhalidwe kuli kochepa kwambiri, kukula kwa mycelium kumachedwa;Kutentha kukakhala kokwera kwambiri, ma pellets a mycelium anali otayirira komanso ochepa, ndipo mphamvu ndi mtundu wa ma pellets a mycelium zidachepa.

Kugwedezeka pafupipafupi komanso kutulutsa kwabwino kwa botolo: bowa wodyedwa ndi bowa wa aerobic, chikhalidwe chamadzimadzi, makamaka ndi kuyamwa kwa okosijeni wosungunuka mu chikhalidwe cha sing'anga.The kusungunuka mpweya mu sing'anga chikhalidwe makamaka amakhudzidwa ndi mamasukidwe akayendedwe a sing'anga, kuchuluka kwa madzi mu chidebe, pafupipafupi oscillation ndi zinthu zina.Kugwedeza kwafupipafupi kumakhala kwakukulu, botolo logwedezeka ndi laling'ono, kuchuluka kwa sing'anga mpaka, mpweya wosungunuka wa sing'anga ndi wapamwamba, ndipo njira ina yozungulira ndi yochepa.Nthawi zambiri kuthamanga kwa rotary shaker ndi 180-220 RPM / min, kubwereza ndi 80-120 RPM / min, matalikidwe 6-7cm.

Ph of chikhalidwe sing'anga: PH ya sing'anga ya chikhalidwe imakhudza mwachindunji kuyamwa kwa michere, zochitika za enzyme ndi kukula kwa mycelial pellet.PH yeniyeni iyenera kusinthidwa musanatseke, bowa wodyedwa kwambiri mu pH 2.0-6.0.Pofuna kupewa kusintha kwakukulu kwa PH mu chikhalidwe cha chikhalidwe, calcium carbonate, phosphate ndi zinthu zina zowonongeka nthawi zambiri zimawonjezeredwa ku chikhalidwe cha chikhalidwe.

Kukhuthala kwapakatikati: Kukhuthala kwapakatikati kumakhudza mwachindunji kuchuluka kwa okosijeni wosungunuka mmenemo, komanso kumakhudza mapangidwe a mycelial pellets.Zotsatirazo zinasonyeza kuti pamene kukhuthala kwa chikhalidwe cha chikhalidwe kumawonjezeka, kukula kwa ma pellets a mycelium kunachepa, chiwerengero chikuwonjezeka, ndipo zokolola zimawonjezeka.Chifukwa chake, malinga ndi zofunikira zamadzimadzi amadzimadzi pamimba ya mycelium pellets, sing'anga yachikhalidwe yokhala ndi mamasukidwe enaake iyenera kukhazikitsidwa.Chikhalidwe cha ma cell ndi ntchito yovuta, makamaka ikafunika kutukuka mothandizidwa ndi shaker, monga shaker yochita bwino kwambiri, iyenera kuganiziridwa mozama, kuti zitsimikizire kuti chikhalidwe cha cell chikuyenda bwino.