Cell fakitale ndi wamba consumable mu lalikulu lonse chikhalidwe cell, amene makamaka adherent cell chikhalidwe.Kukula kwa maselo kumafunikira mitundu yonse ya zakudya, ndiye ndi chiyani?
1. Sing'anga ya chikhalidwe
Selo chikhalidwe sing'anga amapereka maselo mu selo fakitale ndi zakudya zofunika kuti kukula, kuphatikizapo chakudya, amino zidulo, mchere mchere, mavitamini, etc. Pali zosiyanasiyana zopangira zoulutsira kupezeka kwa zakudya zosowa za maselo osiyanasiyana, monga EBSS. , Mphungu, MEM, RPMll640, DEM, etc.

2. Zina zowonjezera zowonjezera
Kuphatikiza pa zakudya zofunikira zomwe zimaperekedwa ndi ma media osiyanasiyana opangira, zigawo zina, monga seramu ndi zinthu, ziyenera kuwonjezeredwa molingana ndi maselo osiyanasiyana komanso zolinga zachikhalidwe.
Seramu imapereka zinthu zofunika monga matrix a extracellular, kukula ndi transferrin, ndi seramu ya fetal bovine imagwiritsidwa ntchito kwambiri.Kuchuluka kwa seramu yoti iwonjezedwe kumadalira selo ndi cholinga cha phunzirolo.10% ~ 20% seramu akhoza kukhalabe kukula mofulumira ndi kuchuluka kwa maselo, wotchedwa sing'anga kukula;Pofuna kusunga kukula pang'onopang'ono kapena kusafa kwa maselo, 2% ~ 5% seramu ikhoza kuwonjezeredwa, yotchedwa chikhalidwe chokonzekera.
Glutamine ndiye gwero lofunikira la nayitrogeni pakukula kwa ma cell ndipo imagwira ntchito yofunika kwambiri pakukula kwa maselo ndi metabolism.Komabe, chifukwa glutamine ndi yosakhazikika komanso yosavuta kusokoneza yankho, imatha kuwola pafupifupi 50% patatha masiku 7 pa 4 ℃, kotero glutamine iyenera kuwonjezeredwa musanagwiritse ntchito.
Nthawi zambiri, ma media osiyanasiyana ndi seramu amagwiritsidwa ntchito mu chikhalidwe cha ma cell, koma pofuna kupewa kuipitsidwa kwa maselo pachikhalidwe, kuchuluka kwa maantibayotiki, monga penicillin, streptomycin, gentamicin, ndi zina zambiri, amawonjezeredwa pawailesi.