• labu-217043_1280

Kodi kusankha cell chikhalidwe consumables ?

1. Dziwani njira yolima

Malingana ndi njira zosiyanasiyana za kukula, maselo amagawidwa m'magulu awiri: maselo otsatizana ndi maselo oyimitsidwa, ndipo palinso maselo omwe amatha kukula motsatira ndi kuyimitsidwa, monga maselo a SF9.Maselo osiyanasiyana ali ndi zofunikira zosiyanasiyana zama cell chikhalidwe consumables.Maselo otsatizana nthawi zambiri amagwiritsa ntchito zinthu zomwe zimathandizidwa ndi TC, pomwe ma cell oyimitsidwa alibe zofunikira zotere, koma zogwiritsidwa ntchito ndi TC ndizoyeneranso kuyimitsidwa kwa cell.Kuti musankhe zogwiritsira ntchito zoyenera, njira ya chikhalidwe cha selo iyenera kutsimikiziridwa molingana ndi mtundu wa selo.

2. Sankhani mtundu wa consumables

Zomwe zimagwiritsidwa ntchito pagulu la ma cell zimaphatikizira mbale za chikhalidwe cha ma cell, mbale za cell culture, cell culture square square, botolo la cell roller, mafakitale a cell,Serological pipettes, ndi zina zotero. Zogwiritsidwa ntchitozi zimakhala ndi makhalidwe awoawo malinga ndi chikhalidwe cha chikhalidwe, njira yogwiritsira ntchito, ndi dongosolo lonse.Botolo la chikhalidwe ndi chikhalidwe chotsekedwa, chomwe chingachepetse kuipitsa;chikhalidwe mbale ndipetri mbalendi chikhalidwe chotseguka, chomwe chimakhala chosavuta kuyesa kuyesa ndi kuyesa kwa gradient, koma chimakhalanso choyambitsa kuipitsidwa ndi mabakiteriya, omwe amafunikira oyendetsa apamwamba.Zina zowonjezera zimafunikanso kugwiritsidwa ntchito ndi zida zapadera.Mwachitsanzo, chogwedeza ma cell chimafunika kugwiritsa ntchito kugwedezeka kwa chogwedezacho kuti ma cell agwirizane bwino ndi mpweya, ndipo fakitale yama cell 40 imafunikira zida zodziwikiratu.Mwachidule, posankha mtundu wa zogwiritsidwa ntchito, ziyenera kuganiziridwa mozama pamodzi ndi zosowa zoyesera komanso zokonda zaumwini.

1.Multi-chabwinoMa cell Culture Plates: Mawonekedwe a chikhalidwe cha ma cell omwe amagwiritsa ntchito mbale zokhala ndi ma cell ambiri ayamba kutchuka chifukwa amathandizira kuphunzira zamitundu yosiyanasiyana, amachepetsa nthawi yoyesera, ndikusunga zopangira zokwera mtengo.Kuphatikiza pa ma mbale ang'onoang'ono apamwamba kwambiri, mbale zazing'ono zapadera zapangidwa kuti zithandizire 3D ndi chikhalidwe cha cell organotypic.

1) Chiwerengero cha mabowo

Zimatengera mulingo womwe mukufuna, komanso popanda thandizo la makina.6, 12, 24 ndi mbale zina zocheperako bwino zama cell zitha kuwonjezeredwa pamanja.Ku 96 - chabwinomaselo chikhalidwe mbale, ndi bwino kukhala ndi chithandizo cha pipette yamagetsi kapena makina.

2) Maonekedwe a dzenje

Pansi pa chitsimecho chikhoza kusankhidwa kukhala chathyathyathya (F-pansi), kuzungulira (U-pansi), kapena chojambulidwa, kutengera mtundu wa selo ndi kugwiritsa ntchito pansi.

3) Mtundu wa mbale

Mtundu wa mbale ya perforated umagwirizananso kwambiri ndi ntchito.Ngati ma cell awonedwa ndi gawo losiyanitsa ndi maikulosikopu kapena ndi maso, mbale yowoneka bwino yama cell imatha kusankhidwa.Komabe, pazogwiritsa ntchito kunja kwa kuwala kowoneka bwino (monga luminescence kapena fluorescence), zokhala ndi mitundu yambiri.maselo chikhalidwe mbale(monga zoyera kapena zakuda) zimafunika.

4) Chithandizo chapamwamba

Ndi chithandizo chamtundu wanji chomwe mungasankhe chimadalira ngati mukukulitsa kuyimitsidwa kapena ma cell omvera.

2.Mabotolo a chikhalidwe cha ma cell: Dera la chikhalidwe limachokera ku 25-225 cm², ndipo nthawi zambiri amasinthidwa pamwamba, oyenera kumamatira ndi kukula.225cm² ndi 175cm²ma cell culture flasksamagwiritsidwa ntchito kwambiri pazikhalidwe zazikulu (monga chikhalidwe cha cell monoclonal, etc.), 75cm² imagwiritsidwa ntchito kwambiri poyesa ma cell (ndimeyi, kusungidwa kwa maselo, maselo oyesera, ndi zina), 25cm² imagwiritsidwa ntchito kwambiri kugwiritsidwa ntchito kutsitsimutsa maselo kapena chikhalidwe pamene pali maselo ochepa, ndipo popanga maselo oyambirira, mabotolo angapo angagwiritsidwe ntchito kuti apewe kuipitsidwa.

3.Botolo la Erlenmeyer: Poyerekeza ndi zinthu zomwe zimagwiritsidwa ntchito monga mafakitale a cell ndi botolo la cell roller, ili ndi malo ang'onoang'ono a chikhalidwe cha maselo ndipo ndi chida chachuma cha chikhalidwe cha maselo.Thupi la botolo la botolo limapangidwa ndi polycarbonate (PC) kapena PETG zakuthupi.Mapangidwe apadera a mawonekedwe a katatu amachititsa kuti pipette kapena cell scraper ifike pakona ya botolo, zomwe zimapangitsa kuti ntchito ya chikhalidwe cha selo ikhale yosavuta.Thebotolo la erlenmeyerkapu imapangidwa ndi zinthu zamphamvu kwambiri za HDPE, zomwe zimagawidwa kukhala chipewa chosindikizira ndi kapu yopumira.Chophimba chosindikizira chimagwiritsidwa ntchito pachikhalidwe chosindikizidwa cha gasi ndi madzi.Chophimba chopumira chimakhala ndi nembanemba ya hydrophobic pamwamba pa kapu ya botolo.Zimalepheretsa kulowa ndi kutuluka kwa tizilombo tating'onoting'ono, zimalepheretsa kuipitsa, komanso zimatsimikizira kusinthanitsa gasi, kotero kuti maselo kapena mabakiteriya amakula bwino.

Miyezo yodziwika bwino ya conical kugwedezekaerlenmeyer botolondi 125ml, 250ml, 500ml, 1000ml ndi3L, 5L mkulu dzuwa erlenmeyer flasks, Kuti muwone mphamvu ya sing'anga ndikumvetsetsa kukula kwa maselo, sikelo idzasindikizidwa pa thupi la botolo.Chikhalidwe cha ma cell chiyenera kuchitidwa m'malo owuma.Choncho, botolo la Erlenmeyer lidzapatsidwa chithandizo chapadera choletsa kubereka musanagwiritse ntchito kuti akwaniritse zotsatira za palibe DNase, palibe RNase, komanso zosakaniza zochokera ku zinyama, zomwe zimapereka mikhalidwe yabwino ya kukula kwa maselo.malo ozungulira.

4.Multi-layerMa cell Factory: Fakitale ya ma cell ndi Yoyenera kupanga ma batch a mafakitale, monga katemera, ma antibodies a monoclonal kapena makampani opanga mankhwala, komanso ntchito za labotale ndi chikhalidwe chachikulu cha cell.Zosavuta komanso zothandiza, zimapewa bwino kuipitsa.Cell Factory yokhala ndi Chivundikiro Chosindikizidwa: Chivundikirocho chilibe mabowo olowera mpweya, ndipo chimagwiritsidwa ntchito makamaka pansi pamikhalidwe yopanda mpweya woipa monga zofungatira ndi nyumba zobiriwira.Cell Factory yokhala ndi chivundikiro chotsekedwa imatha kuletsa kuukira kwa mabakiteriya akunja ndikuthandizira kupanga malo abwino oti kukula kwa maselo.Chivundikiro chopumira mpweya: Pamwamba pa chivundikirocho pali mabowo olowera mpweya, omwe amagwiritsidwa ntchito makamaka pamalo a carbon dioxide.Mabowo olowera mpweya amalola mpweya woipa m'chilengedwe kulowa mufakitale yama cell, ndikupanga mikhalidwe yoyenera kukula kwa cell.Pali 1 layer,2 layers,5 layers,10 layers,40 layersmafakitale a cellkupezeka.

5.Cell chikhalidwebotolo lodzigudubuza: Mabotolo odzigudubuza a 2L & 5L Ndi Oyenera ku mitundu yosiyanasiyana ya chikhalidwe cha maselo ndi chikhalidwe cha Suspension cell, kuphatikizapo maselo a Vero, maselo a HEK 293, maselo a CAR-T, MRC5, maselo a CEF, macrophages a porcine alveolar, maselo a myeloma, maselo a DF-1, Ma cell a ST, ma cell a PK15, ma cell a Marc145 ena omwe amatsatira.Ndiwoyeneranso chikhalidwe chosasunthika cha maselo oyimitsidwa monga ma cell a CHO, maselo a tizilombo, maselo a BHK21 ndi maselo a MDCK.

3.Sankhani zofunikira za consumables. 

Kuyesera kwakukulu kwa chikhalidwe cha maselo kumafuna zogwiritsidwa ntchito ndi malo akuluakulu a chikhalidwe kuti athandizidwe, pamene zoyesera zazing'ono zimasankha zogwiritsira ntchito ndi malo ang'onoang'ono.Mafakitale a ma cell amagwiritsidwa ntchito kwambiri pazachikhalidwe chachikulu cha maselo, monga kupanga katemera, ma antibodies a monoclonal, makampani opanga mankhwala, ndi zina zambiri;mbale zachikhalidwe, mbale, ndi ma flasks ndi oyenera kutengera chikhalidwe chaching'ono cha cell m'ma laboratories;kuwonjezera kuyimitsidwa selo chikhalidwe, botolo angathenso Pakuti sing'anga kukonzekera, kusakaniza ndi kusunga.Malinga ndi kuchuluka kwa chikhalidwe cha cell, dziwani zomwe zimagwiritsidwa ntchito.

Zogwiritsira ntchito zama cell zoyenerera ndizo maziko owonetsetsa kukula kwa maselo abwino, komanso ndi chinsinsi chofulumizitsa njira yoyesera ndikuwonetsetsa chikhalidwe cha chikhalidwe.Pakusankhidwa, zinthu monga njira ya chikhalidwe cha ma cell, kukula kwa chikhalidwe, ndi ma labotale ziyenera kuganiziridwa mozama.tiyenera kugwiritsa ntchito zina consumable pamene kupanga selo chikhalidwe, mwachitsanzo,chonyamulira cha CellDisk&chonyamulira chozungulira cha CellDisk,malangizo a pipette,filimu yosindikizira,mapaipi, etc., Luoron angaperekenso.

LuoRon Biotech Co., Ltd imayang'ana kwambiri pa kafukufuku, chitukuko, kugulitsa ndi ntchito zazachilengedwe.Fakitale yopanga ili ndi malo obzala 10,000 masikweya mita.Ili ndi kalasi ya 100,000 yopanga ukhondo, msonkhano wapagulu wa giredi 10,000 komanso kafukufuku wapamwamba kwambiri wa nkhungu ndi msonkhano wopanga.

Mwachidule, posankha mtundu wa zogwiritsidwa ntchito, ziyenera kuganiziridwa mozama pamodzi ndi zosowa zoyesera komanso zokonda zaumwini.Zachidziwikire, ndikofunikira kusankha nsanja ngati LuoRon yomwe ili ndi zinthu zapamwamba komanso zosiyanasiyana, zokhazikika, zotsimikizika komanso ntchito.LuoRon atha kupereka ntchito zosiyanasiyana zogulira zinthu kamodzi kokha zopangira kafukufuku wasayansi m'ma laboratories okhudzana ndi sayansi ya moyo wapadziko lonse lapansi, makampani opanga mankhwala, kuteteza chilengedwe, chitetezo cha chakudya, mabungwe aboma, ndi zamankhwala azachipatala.

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