Chikhalidwe cha ma cell ndi njira yoti ma cell apulumuke, akule, aberekane komanso asungire zomanga ndi ntchito zawo zazikulu potengera chilengedwe mu vivo mu vitro.Selo chikhalidwe botolondi mtundu wa cell consumable ntchito adherent cell chikhalidwe.M'kati mwa cell chikhalidwe, nthawi zambiri timapeza zonyansa zina kudzikundikira madzi.Pali zifukwa zambiri za izi, ndipo kutentha ndi chimodzi mwa zifukwa zofala.
Kukhalapo kwa mvula mu botolo la chikhalidwe cha ma cell kungakhale chifukwa cha kuipitsidwa kwa ma cell.Ngati kuipitsidwa sikukuphatikizidwa, turbidity mu cell culture medium nthawi zambiri imatanthauzidwa ngati mvula yazinthu zachitsulo, mapuloteni, ndi zigawo zina zapakati.Ambiri precipitates amawononga yachibadwa maselo kuchuluka chifukwa amasintha zikuchokera sing'anga ndi chelating zakudya ndi zina zofunika zigawo zikuluzikulu.Kuthamanga kumatha kuwonedwa mwachisawawa ndipo kungasokoneze zoyeserera zomwe zimafunikira kusanthula kwazithunzi.
 
Mu chikhalidwe cha ma cell, kutentha ndi chimodzi mwazinthu zazikulu zomwe zimayambitsa mvula.Kutentha kukakhala kotentha kwambiri, mapuloteni a plasma olemera kwambiri amapangidwa kuchokera ku yankho.Kutentha kosasunthika komanso kuzizira kungathe kulimbikitsa kuwonongeka kwa mapuloteni ndi mpweya.Chifukwa madzi kapena sing'anga yokonzedwanso imasungidwa kumalo ozizira pakati pa kugwiritsidwa ntchito, mchere ukhoza kukhazikika, makamaka mu 10X kapena njira zina zosungiramo zosungirako.
 
Zachidziwikire, mvula imawonekera mu botolo la chikhalidwe cha ma cell.Ngati zatsimikiziridwa kuti kutentha ndi chifukwa, chidwi ayenera kuperekedwa kwa malo yosungirako ndi ntchito njira ya sing'anga chikhalidwe kupewa kuzizira ndi thawing mobwerezabwereza, zomwe zingachepetse mwayi wa mvula.