Pali zidutswa zambiri zama cell pambuyo pa makina lysis kuyimitsidwa kwa selo.Kodi kuchotsa zidutswa izi?Tiyeni tiwone njira zosiyanasiyana:

1. Gwiritsani ntchito njira ya dilution.Maselo akachepetsedwa, amathanso kuchulukana, amachulukirachulukira, ndipo zinyalala zama cell zimacheperachepera.
2. Palinso kukhazikika mwachilengedwe.Maselo amakhazikika mwachangu kuposa tizidutswa tambiri: : Sunthani kuyimitsidwa kwa selo muchubu cha centrifuge, ndipo pamene maselo ambiri amira, yankho lapamwamba likhoza kuyamwa, ndikuwonjezeredwa ku njira yothetsera chikhalidwe kuti ayimitse maselo.Njirayi ingagwiritsidwe ntchito mobwerezabwereza, pamene nthawi iliyonse mukhoza kuona ngati ikukwaniritsa zofunikira zanu pansi pa microscope.
3. Low-liwiro centrifugeakhoza kuchotsa zinyalala, zambiri 700g, 5mins
4. Pamene centrifuging, pansi pa chikhalidwe chakuti chiwerengero cha maselo ndi chokwanira, kuchepetsa nthawi ya centrifugation, monga 5min, 1000rpm, m'malo mwa 3min, 1000rpm, ndi kuchotsa supernatant, chifukwa necrosis ndi zinyalala zambiri mu supernatant!Opaleshoniyi imangofunika kuchitidwa kamodzi panthawi yotsuka musanalowetse!

Ngakhale pali njira zambiri zochotsera zinyalala zama cell, mupeza kuti centrifugation ndi imodzi mwa njira zabwino kwambiri!

Ndipo chifukwa minyewa ya zomera ndi ma cell ali ndi makoma a cellulose, hemicellulose ndi pectin, nthawi zambiri ndikofunikira kugaya mchenga wa quartz kapena ufa wagalasi ndi yankho loyenera lochotsa kapena kuthira ndi cellulase kuti akwaniritse cholingacho.Kugawikana kwa maselo a bakiteriya kumakhala kovuta kwambiri, chifukwa mafupa onse a bakiteriya amalumikizana ndi ma peptidoglycan cystic macromolecules, olimba kwambiri.Njira zodziwika bwino zothyola makoma a ma cell a bakiteriya ndi monga kuphwanya kwa akupanga, kugaya mchenga, kutulutsa mphamvu kwambiri kapena mankhwala a lysozyme.Minofu ndi ma cell zikasweka, chotchinga choyenera chimasankhidwa kuti chichotse puloteni yomwe mukufuna.Zinthu zosasungunuka monga zidutswa zama cell zimachotsedwa ndicentrifugekapena kusefera.

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